PCR-Reverse Line Blot Typing Method Underscores the Genomic Heterogeneity of Borrelia valaisiana Species and Suggests Its Potential Involvement in Lyme Disease
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چکیده
منابع مشابه
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Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR products using streptavidin-peroxidase...
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Genetic and phenotypic analysis of Borrelia valaisiana sp. nov. (Borrelia genomic groups VS116 and M19).
To clarify the taxonomic status of two recently described Borrelia genomic groups, groups VS116 and M19, three group VS116 strains and eight group M19 strains isolated from Ixodes ricinus ticks in Switzerland, The Netherlands, and the United Kingdom were characterized. PCR-restriction fragment length polymorphism (RFLP) analysis of the 5S-23S intergenic spacer amplicon, rRNA gene restriction an...
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sive alternative to glycopeptides for the treatment of MRSA infections. Data from the United States and Europe have shown that vancomycinintermediate S. aureus isolates also remain susceptible to some of the conventional antimicrobial drugs, including co-trimoxazole (5). If their efficacy in vivo is validated by clinical trials, use of these conventional drugs would not only reduce the load on ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2003
ISSN: 0095-1137
DOI: 10.1128/jcm.41.8.3690-3698.2003